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The physical, chemical, and microbiological stability of chloramphenicol ophthalmic solution

1 Department of Basic Sciences and Pharmaceutics, University of Benghazi, Benghazi, Libya
2 Faculty of Pharmacy, University of Benghazi, Benghazi, Libya

Correspondence Address:
Idris Mohamed El-Mahdi,
Department of Pharmaceutics, Faculty of Pharmacy, University of Benghazi, P.O. Box 5341, Benghazi
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2519-139x.237043

Aims: The aim of this work is to “predict” the remaining shelf-life of chloramphenicol (CH) eye drops, commercially marketed, using the theoretical “Longland–Rowbotham model,”” followed by confirmation of findings by practical means. Materials and Methods: The methods used for the evaluation of CH-eye drops included the assay of the active ingredient, sterility testing, and minimum inhibitory concentration (MIC) determination using official methods during 6 months stability study at variable temperatures (4, 25°C). Furthermore, a 3-month accelerated stability study was carried out. Statistical analysis tests included Student's t-test and analysis of variance. Results: The prediction model indicated that in pessimistic conditions, the remaining shelf life was reduced to a merely 1 month following production (this is versus the 2 years expiration date given by manufacturer). However, the samples analyzed throughout a 6-month stability study revealed that storing CH solution at 4°C or 25°C does not produce any statistical difference regarding drug content, MIC, or sterility. Accelerated stability studies for 3 months period showed that only after 2 months from storage at 55°C the drug will start to degrade and a statistical difference could be observed. Conclusion: Storing CH-eye drops at room temperature for up to 6 months appears to have no effect on the stability of this antibiotic.

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