|Year : 2018 | Volume
| Issue : 1 | Page : 3-7
Effects of the aqueous extract from Abelmoschus esculentus L peel on hyperglycemia and hyperlipidemia induced by dexamethasone in rats
Aisha Mohamed Dugani1, Wesal Issa Alkhetally1, Elham Omran Elghedafi2, Feras Walid Alkayed1
1 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Tripoli, Tripoli, Libya
2 Department of Cardiology, Tripoli Medical Center, Tripoli, Libya
|Date of Submission||22-Nov-2017|
|Date of Acceptance||24-Jan-2018|
|Date of Web Publication||05-Apr-2018|
Prof. Aisha Mohamed Dugani
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, University of Tripoli, Tripoli
Source of Support: None, Conflict of Interest: None
Background: Hyperglycemia and hyperlipidemias are common clinical problem among users of glucocorticoids (GCs). The aim of the present study was to explore the effect of oral administration of the aqueous extract of Abelmoschus esculentus peel (AEPE) on hyperglycemia and hyperlipidemia induced in rats by dexamethasone (DEXA). Methods: Twenty-four rats were randomly divided into four equal groups. Each group was treated for 10 days either with 2% carboxymethylcellulose orally (normal control); 10 mg/kg DEXA subcutaneously (hyperglycemic group); 100 mg/kg AEPE orally plus 10 mg/kg DEXA subcutaneously (treatment group 1); or 200 mg/kg AEPE orally plus 10 mg/kg DEXA subcutaneously (treatment group 2). Animals were killed after 10 days of treatments by decapitation, their blood collected for the analysis of blood sugar and lipid profile. Results: Treatment with DEXA induced a significant increase in blood glucose and all lipids and a significant reduction in body weights. After 10 days of treatment, 100 mg/kg of AEPE was able to significantly reduce the effect of DEXA on triglycerides and low-density lipoprotein (LDL) only. 200 mg/kg of AEPE was able to significantly reduce the effect of DEXA on blood glucose levels, cholesterol, triglycerides, and LDL. Both doses of AEPE were able to increase high-density lipoprotein. Conclusion: This study suggests that the AEPE could be beneficial in protecting against GC-induced hyperglycemia and hyperlipidemia.
Keywords: Abelmoschus esculentus, dexamethasone, hyperglycemia, hyperlipidemia, okra
|How to cite this article:|
Dugani AM, Alkhetally WI, Elghedafi EO, Alkayed FW. Effects of the aqueous extract from Abelmoschus esculentus L peel on hyperglycemia and hyperlipidemia induced by dexamethasone in rats. Libyan Int Med Univ J 2018;3:3-7
|How to cite this URL:|
Dugani AM, Alkhetally WI, Elghedafi EO, Alkayed FW. Effects of the aqueous extract from Abelmoschus esculentus L peel on hyperglycemia and hyperlipidemia induced by dexamethasone in rats. Libyan Int Med Univ J [serial online] 2018 [cited 2019 Apr 19];3:3-7. Available from: http://journal.limu.edu.ly/text.asp?2018/3/1/3/229381
| Introduction|| |
Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. Hyperlipidemia is a common manifestation of type 1 and type 2 diabetes and imposes a high risk for cardiovascular diseases. In Libya, many patients with diabetes, in addition to medical therapy, are using several natural products as remedies.Abelmoschus esculentus (AE) (L.) Moench., synonym of okra, also known as lady's fingers or gumbo is a flowering plant from the mallow family. It is valued for its edible green seed pods. It is an important vegetable and widely distributed from Africa to Asia, Southern Europe, and America. The fibers in okra were suggested to help in stabilizing blood sugar by regulating the rate of sugar absorption from the intestinal tract normalizing blood sugar and cholesterol levels., Previous studies reported that the polysaccharide in okra possesses anticomplementary and hypoglycemic activity in normal mice  and can lower cholesterol level in blood by its ability to bind bile acids. Sabitha et al. have found that oral administration of the aqueous extract of AE (L.) produced a significant hypoglycemic effect in streptozotocin-induced diabetes mellitus in rats and with a significant reduction in lipid profile compared to diabetic control group. The extract of AE per se was also found to possess a hypoglycemic effect in mice with no observable changes in behavior.
Dexamethasone (DEXA) is a very potent and highly selective glucocorticoid (GC) having profound anti-inflammatory and immunosuppressive properties that are critical for the treatment of rheumatoid arthritis, cerebral edema, allergic reactions, asthma, and certain types of cancer. Unfortunately, the development of major metabolic side effects remains the key limitation for the long-term use of GCs. Common side effects requiring dosage adjustment or cessation of treatment include diabetes, hypertension, osteoporosis, and muscle wasting. The odds ratio for new-onset diabetes mellitus in GC-receiving patients ranges from 1.5 to 2.5 and the risk increases proportionally with increasing the GC dosage.
Therefore, this study was aimed to investigate the protective effect of AE peel extract in rats against DEXA-induced hyperglycemia and hyperlipidemia.
| Materials and Methods|| |
Male Wistar albino rats weighing between 150 and 250 g were used. The animals were housed in the animal care facility in the Department of Pharmacology and Clinical Pharmacy and maintained at 23°C with a 12:12 h light: Dark cycle. Animals were kept fasting, overnight, 1 day before starting the experiment.
Plant material and preparation of the aqueous extract
AE was collected from the local market, Tripoli, Libya. The pods were thoroughly washed with tap water and then with distilled water. Seeds were removed, and the peel was dried under shade for 2 weeks, made into coarse powder in a grinder and was stored in an airtight container, up to the completion of the study. Fresh solutions were prepared using 2% carboxymethylcellulose (CMC) as vehicle to disperse the powder of AE.
Drugs and chemicals
DEXA sodium phosphate (Dexone ®, Dorcas pharmaceutical laboratories, Sousse, Tunisia), glibenclamide tablet (Gliboral, Menarini International., Pisa, Italy), CMC and diethyl ether were obtained from BDH Chemicals Ltd., Poole, England).
Induction of hyperglycemia and hyperlipidemia
Hyperglycemia and hyperlipidemia were done by subcutaneous administration of DEXA (10 mg/kg for 10 days) according to the procedure described by Shalam et al.
Animals were fasted overnight before starting the experiments. Rats were weighed and then were divided into four groups of six rats each. Group 1 is the normal control received 2% CMC (5 ml/kg/day for 10 days) orally. Group 2: (DEXA-treated group) received 10 mg/kg DEXA subcutaneously for 10 days. Group 3: (treatment group 1) received 100 mg/kg A. esculentus peel extract (AEPE) orally along with 10 mg/kg DEXA subcutaneously for 10 days. Group 4: (treatment group 2) received 200 mg/kg AEPE orally along with 10 mg/kg DEXA subcutaneously for 10 days.
After treatment period, body weight was measured, and animals were sacrificed by decapitation, their trunk blood was collected directly into a centrifuge tube and allowed to clot for 45–60 min at room temperature. Serum was separated by centrifugation at 2500 rpm for 15 min and analyzed for the following biochemical parameters: total cholesterol (TC), triglycerides (TG) not triglycerides (GL), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) by Cobas integra ® 400 (Roche, Switzerland) using commercial kits (Roche-Cobas, Switzerland) according to the manufacturer's protocol. Blood glucose levels were measured using Oncall ® Plus glucometer with the corresponding Oncall ® test strips, (Acon laboratories, Inc., San Diego, CA, USA).
The values were expressed as mean ± standard error. Data were analyzed using SPSS version 17.0 (SPSS Inc., USA). The differences between groups were analyzed using unpaired Student's t-test. P < 0.05 was considered statistically significant.
| Results|| |
A significant reduction in body weight was observed in the DEXA-treated group, and AEPE (200 mg/kg) plus DEXA-treated group compared to vehicle control group [Table 1].
|Table 1: Effect of the aqueous extract of Abelmoschus esculentus peel on body weight, fasting blood glucose, and lipid profile|
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Animals treated with DEXA only showed a significant increase in fasting blood glucose levels (132.71%) compared to vehicle control group. Oral administration of 200 mg/kg of AEPE reduced significantly blood glucose levels to 76.24% of DEXA-treated group [23.76% - [Table 1].
DEXA produced a significant increase in TC, TG, HDL, and LDL when compared to control vehicle-treated group. A total of 100 mg/kg of AEPE was able significantly to reduce the effect of DEXA on TG and LDL while potentiating its effect on HDL. On the other hand, 200 mg/kg of AEPE was able to antagonize the effect of DEXA on TC, TG, and LDL, while potentiating its effect on HDL [Table 1].
| Discussion|| |
DEXA is a potent GC with many therapeutic applications. High exposure to GCs impairs insulin sensitivity, contributing to the generation of metabolic syndrome including insulin resistance, and hypertension. DEXA also increases TG causing an imbalance in lipid metabolism leading to hyperlipidemia  and increases glucose levels leading to hyperglycemia. These serious side effects of GCs require special attention and limit their clinical applications.
AE (Okra) is a popular healthy food due to its high fiber, Vitamin C, and folate contents. Okra is also known for being high in antioxidants and is a good source of calcium and potassium. It was also reported that AEPE possesses hypoglycemic effect against streptozotocin and alloxan-induced diabetes in rats., In view of the forwarded mentioned facts, we decided to examine the possibility of a protective effect of AEPE against the hyperlipidemias and hyperglycemia induced by the DEXA in rats.
Administration of DEXA for 10 days increased levels of glucose, TG, TC, LDL, and HDL accompanied by a decrease in body weights. The high dose of AEPE (200 mg/kg) significantly prevented all the effects of DEXA except those on HDL where it potentiated its effect. One the other hand, the lower dose of AEPE (100 mg/kg) also prevented the effect of DEXA on TG and LDL but potentiated its effect on HDL. Azeez and Kheder  have showed that Gundelia tournefortii reduced blood glucose in DEXA-induced hyperglycemia in mice and attributed this effect to the presence of flavonoids which stimulate insulin secretion. Lin et al. had demonstrated that flowers and fruit of AE were rich in flavonoids. Therefore, it is tempting to speculate that hypoglycemic effect of AEPE in our study may be due to its contents of flavonoids.
Hyperlipidemia is accepted as an independent risk factor for cardiovascular disorders, and therefore, it requires special management. As it has been demonstrated in our study, AEPE especially at the dose of 200 mg/kg significantly reduced blood levels of TC, TG, LDL, and increased the levels of HDL compared to the DEXA-treated group. In support of our findings, Trinh et al. have demonstrated that AEPE reduced cholesterol and triglycerides levels in mice made hyperlipidemic by intraperitoneal administration of a single dose of tyloxapol. Moreover, in invitro study, it has been shown that okra is able to bind bile acids and hence may reduce their reabsorption from GIT. Thus, the observed effects in our study may be due to ability of AEPE to reduce absorption of cholesterol from the diet or by interfering with cholesterol synthesis in the liver.
| Conclusion|| |
This study suggests that the aqueous extract of A. esculentus L can be considered as a preventive therapy against GC-induced hyperglycemia and hyperlipidemia in rats as demonstrated by its ability to normalize blood glucose levels and its positive effect on lipid profile.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2009;32 Suppl 1:S62-7.
El-Ghadi A, Bshana S. Usage of some plants in Libyan folk medicine, Part 1 and 2. Dar Elkotob Elwataniya; 1988.
Khomsug P, Thongjaroenbuangam W, Pakdeenarong N, Suttajit M, Chantiratikul P. Antioxidative activities and phenolic content of extracts from Okra (Abelmoschus esculentus
L.). Res J Biol Sci 2010;5:310-3.
Sengkhamparn N, Verhoef R, Schols HA, Sajjaanantakul T, Voragen AG. Characterisation of cell wall polysaccharides from okra (Abelmoschus esculentus
(L.) moench). Carbohydr Res 2009;344:1824-32.
Tomoda M, Shimizu N, Gonda R, Kanari M, Yamada H, Hikino H, et al.
Anticomplementary and hypoglycemic activity of okra and hibiscus mucilages. Carbohydr Res 1989;190:323-8.
Kahlon TS, Chapman MH, Smith GE.In vitro
binding of bile acids by okra, beets, asparagus, eggplant, turnips, green beans, carrot and cauliflower. Food Chem 2007;103:676-80.
Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K. Antidiabetic and antihyperlipidemic potential of Abelmoschus esculentus
(L.) moench. In streptozotocin-induced diabetic rats. J Pharm Bioallied Sci 2011;3:397-402.
Perez JR, Baritua RJ, Pacalna MO, Malayao SO. Exploratory investigation on the hypoglycemic effect of Abelmoschus esculentus
in mice. IJSTR 2013;2:250-3.
Bernard P, Schimmer BP, Keith LP. Adrenocorticotropic hormone; adrenocortical steroids and their synthetic analogues; inhibitors of the synthesis and actions of adrenocortical hormones. In: Brunton LL, Lazo JS, Parker KL. Goodman & Gillman's, The Pharmacological Basis of Therapeutics. USA: McGraw Hill; 2006. p. 1597-8.
Rosen J, Miner JN. The search for safer glucocorticoid receptor ligands. Endocr Rev 2005;26:452-64.
Clore JN, Thurby-Hay L. Glucocorticoid-induced hyperglycemia. Endocr Pract 2009;15:469-74.
Shalam MD, Harish MS, Farhana SA. Prevention of dexamethasone- and fructose-induced insulin resistance in rats by SH-01D, a herbal preparation. Indian J Pharmacol 2006;38:419-22.
Qi D, Pulinilkunnil T, An D, Ghosh S, Abrahani A, Pospisilik JA, et al.
Single-dose dexamethasone induces whole-body insulin resistance and alters both cardiac fatty acid and carbohydrate metabolism. Diabetes 2004;53:1790-7.
Wiesenberg I, Chiesi M, Missbach M, Spanka C, Pignat W, Carlberg C, et al.
Specific activation of the nuclear receptors PPARgamma and RORA by the antidiabetic thiazolidinedione BRL 49653 and the antiarthritic thiazolidinedione derivative CGP 52608. Mol Pharmacol 1998;53:1131-8.
Mahendran P, Devi CS. Effect of Garcinia cambogia extract on lipids and lipoprotein composition in dexamethasone administered rats. Indian J Physiol Pharmacol 2001;45:345-50.
Roy A, Shrivastava SL, Mandal SM. Functional properties of Okra Abelmoschus esculentus
L. (Moench): Traditional claims and scientific evidences. Plant Sci Today 2014;3:121-30.
Amin IM. Hypoglycemic effects in response to Abelmoschus esculentus
treatment: A Research framework using STZ-induced diabetic rats. Int J Biosci Biochem Bioinform 2011;1:63-7.
Ben-Chioma AE, Tamuno-Emine DG, Dan DB. The effect of Abelmoschus esculentus
in Alloxan-induced diabetic wistar rat. IJSR 2015;4:540-3.
Azeez OH, Kheder AE. Effect of Gundelia tourne fortii
on some biochemical parameters in dexamethasone-induced hyperglycemic and hyperlipidemic mice. Iraqi J Vet Sci 2012;26:73-9.
Lin Y, Lu MF, Liao HB, Li YX, Han W, Yuan K, et al.
Content determination of the flavonoids in the different parts and different species of Abelmoschus esculentus
L. By reversed phase-high performance liquid chromatograph and colorimetric method. Pharmacogn Mag 2014;10:278-84.
Garg A, Grundy SM. Management of dyslipidemia in NIDDM. Diabetes Care 1990;13:153-69.
Trinh HN, Quynh NN, Anh TT, Nguyen VP. Hypolipidemic effect of extracts from Abelmoschus esculentus
L. – Malvaceae on tyloxapol- induced hyperlipidemia in mice. 13rd
International Electronic Conference on Synthetic Organic Chemistry (ECSOC-13); 2009. Available from: http://www. ACER/Downloads/ecsoc-13_216_slides.pdf
. [Last accessed on 2017 Apr 30].